Translational_Unit

Part:BBa_K2848001:Design

Designed by: Alexandra Mack, Benjamin Schaefer   Group: iGEM18_Stuttgart   (2018-10-08)


NodC+COD-HisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The primer for adding the His-Tag was provided with an additional SpeI restriction site.

PCR using BBa_K2380043 as template and the primers VF2 (5'-tgccacctgacgtctaagaa-3') and COD_rev+His6+SpeI (5'-[GCCGCTACTAGTATTAGTGGTGATGGTGATGATG]cagagcagtaaacagcgtattctggg-3').

Source

iGEM Team Darmstadt 2017 (ChiTUcare)

References